It has been recognized that serotonin 2A receptor (5-HT2A) agonist 2,5-dimethoxy-4-iodo-amphetamine (DOI) impairs serotonergic homeostasis. However, the mechanism of DOI-induced serotonergic behaviors remains to be explored. Moreover, little is known about therapeutic interventions against serotonin syndrome, although evidence suggests that ginseng might possess modulating effects on the serotonin system. As ginsenoside Re (GRe) is well-known as a novel antioxidant in the nervous system, we investigated whether GRe modulates 5-HT2A receptor agonist DOI-induced serotonin impairments. We proposed that protein kinase Cδ (PKCδ) mediates serotonergic impairments. Treatment with GRe or 5-HT2A receptor antagonist MDL11939 significantly attenuated DOI-induced serotonergic behaviors (i.e., overall serotonergic syndrome behaviors, head twitch response, hyperthermia) by inhibiting mitochondrial translocation of PKCδ, reducing mitochondrial glutathione peroxidase activity, mitochondrial dysfunction, and mitochondrial oxidative stress in wild-type mice. These attenuations were in line with those observed upon PKCδ inhibition (i.e., pharmacologic inhibitor rottlerin or PKCδ knockout mice). Furthermore, GRe was not further implicated in attenuation mediated by PKCδ knockout in mice. Our results suggest that PKCδ is a therapeutic target for GRe against serotonergic behaviors induced by DOI. 相似文献
The prevention and treatment of erosive tooth wear are becoming increasingly important due to its increasing prevalence. The use of natural solutions to modify dental surfaces has become an area of research. Organic materials such as chitosan and hydrolyzed collagen may be a promising option to treat dentin. This in vitro study aimed to evaluate the influence of chitosan or hydrolyzed collagen, alone or combined with acidulated phosphate fluoride (APF) gel, on the composition and morphology of dentin after erosion. Bovine dentin samples were prepared (n = 84) and treated with artificial saliva (AS, negative control); APF gel (F, positive control); chitosan solution (Chi); hydrolyzed collagen solution (Col); fluoride/chitosan composition (F_Chi); and fluoride/hydrolyzed collagen composition (F_Col). Erosive cycles (six cycles of immersion in orange juice for 1 min, followed by immersion in AS for 1 hr) were performed. The materials were characterized by their morphology, composition, and particle size distribution. Micro-energy dispersive X-ray fluorescence spectroscopy and scanning electron were used to evaluate the dentin's inorganic chemical composition and morphology. The F_Col and F groups had a reduction in calcium loss by 17 and 26%, respectively (p < .001). Both of these groups still had a covering layer of agglomerates at the dentin surface after the erosive cycles. The fluoridated chitosan or collagen solutions improved the dentin resistance to erosion as a novel hybrid-fluoride-based material approach to provide surface protection from erosion. 相似文献
The objective of this study was to evaluate the antimicrobial and antioxidative effects of the onion peels extract prepared
via the subcritical water extraction (SWE) method. First, the number of cells treated with the extract was reduced by 0.7–1.1
log CFU/mL compared with the control. The SWE extract showed 76.08% scavenging activity and it was more effective than butylated
hydroxytoluene (BHT) at 61.3 ppm in lipid peroxidation inhibitory effects. In addition, the antioxidative effect of SWE extract
measured via the ferric thiocyanate (FTC) method was 2-fold that of BHT. The results of this study suggest that the onion
peels extract prepared via the SWE method may have potential alternative antimicrobial and antioxidative effects as functional
substances. 相似文献
This paper presents an optimization of control allocation in integrated chassis control with active front steering, active rear steering, electronic stability control and torque-vectoring device under the saturation of lateral tire forces on front wheels. After a control yaw moment is calculated in the upper-level controller, a weighted pseudo-inverse based control allocation is used for yaw moment generation in the lower-level controller. Variable coefficients of the weighted pseudo-inverse based control allocation are used to represent various actuator combinations and are optimized for each actuator combination to enhance control performances using simulation on vehicle simulation package, CarSim. Due to severe cornering on low friction road, the front lateral tire forces can be easily saturated. Under the condition, the active front steering has little effect on control performance and, consequently, the desired control yaw moment cannot be generated. So, the lateral force generated by AFS should be restricted to its maximum, and a constrained weighted pseudoinverse based control allocation with electronic stability control, active rear steering and torque-vectoring device is applied to compensate the loss of the control yaw moment. Variable coefficients of the constrained weighted pseudo-inverse based control allocation with electronic stability control, active rear steering and torque-vectoring device are also optimized using simulated-based tuning. To validate the proposed method, simulation was done on CarSim. From simulation, it was verified which actuator combination is effective for integrated chassis control if the lateral forces on front wheels are saturated.
A series of nanocomposite polymer electrolytes (NCPEs) comprising nanoparticles of BaTiO3, Al2O3 or SiO2 were prepared by electrospinning technique. The nano-sized ceramic fillers were incorporated into poly(vinylidene fluoride-co-hexafluoropropylene) [P(VdF-HEP)] membranes during the electrospinning process. The resultant porous membranes are good absorbent of the liquid electrolyte and exhibit high electrolyte retention capacity. The presence of the ceramic nanoparticles has positive effect on the mechanical properties of the membranes. The ionic conductivity and the electrochemical stability window of the electrospun P(VdF-HFP)-based polymer are enhanced by the presence of the fillers. The cell Li/LiFePO4 based on the NCPE containing BaTiO3 delivers a discharge capacity of 164 mAh/g, which corresponds to 96.5% utilization of the active material. In comparison, the performance of Li/LiFePO4 cells with NCPEs containing Al2O3 and SiO2 was observed to be lower with respective discharge capacities of 153 and 156 mAh/g. The enhanced performance of the BaTiO3-based-NCPE is attributed mainly to its better interaction with the host polymer and compatibility with lithium metal. 相似文献